mouse anti-e2 antibody  (Abmart Inc)

Journal: Journal of Translational Autoimmunity

Article Title: Development of a standardized monoclonal antibody to the inner lipoyl domain of PDC-E2 as a potential international AMA reference

doi: 10.1016/j.jtauto.2024.100262

Figure Lengend Snippet: Specificity of the 4G6 antibody against PDC-E2 . Cell lysates (10 μg) from Hucc-T1, Hep-G2 and 293T cell line were subjected to 12 % SDS-PAGE gel, transferred to the PVDF membrane, and incubated with 100 ng/mL of the 4G6 antibody (A), Santa Cruz anti-PDC-E2 antibody sc-271534 (B), and a human serum sample (1:1000 dilution) specifically recognizing PDC-E2 (C).

Article Snippet: The 4G6 antibody demonstrated a cytoplasmic staining pattern identical to that observed with a commercial mouse monoclonal anti-PDC-E2 antibody from Santa Cruz.

Techniques: SDS Page, Membrane, Incubation

Journal: Journal of Translational Autoimmunity

Article Title: Development of a standardized monoclonal antibody to the inner lipoyl domain of PDC-E2 as a potential international AMA reference

doi: 10.1016/j.jtauto.2024.100262

Figure Lengend Snippet: Specificity of the 4G6 antibody against inner lipoyl domain of PDC-E2 . Purified 7 pmole of different recombinant proteins were subjected to 12 % SDS-PAGE gel and stained with Coomassie Brillant Blue (A) or transferred to the PVDF membrane and immunoblotted against the 4G6 antibody (B). PDC-E2 (FL), full length PDC-E2; PDC-E2 (ILD + OLD), PDC-E2 with inner and outer lipoyl domains; BPO, recombinant AMA antigen containing only ILD of PDC-E2, and the lipoyl domains of both BCOADC-E2 and OGDC-E2; cTnT, cardiac troponin T. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: The 4G6 antibody demonstrated a cytoplasmic staining pattern identical to that observed with a commercial mouse monoclonal anti-PDC-E2 antibody from Santa Cruz.

Techniques: Purification, Recombinant, SDS Page, Staining, Membrane

Journal: Journal of Translational Autoimmunity

Article Title: Development of a standardized monoclonal antibody to the inner lipoyl domain of PDC-E2 as a potential international AMA reference

doi: 10.1016/j.jtauto.2024.100262

Figure Lengend Snippet: Affinity analysis of chimeric 4G6 antibody with PDC-E2 and BPO . PDC-E2 and BPO proteins were captured to the NTA biosensor at the concentration of 50 μg/mL. The chimeric 4G6 antibody was applied from 1.5625 nM to 12.5 nM. k on is the association rate constant, k off is the dissociation rate constant. The binding affinity constant K D was calculated as k off / k on .

Article Snippet: The 4G6 antibody demonstrated a cytoplasmic staining pattern identical to that observed with a commercial mouse monoclonal anti-PDC-E2 antibody from Santa Cruz.

Techniques: Concentration Assay, Binding Assay

Journal: Journal of Translational Autoimmunity

Article Title: Development of a standardized monoclonal antibody to the inner lipoyl domain of PDC-E2 as a potential international AMA reference

doi: 10.1016/j.jtauto.2024.100262

Figure Lengend Snippet: Immunostaining pattern of the chimeric 4G6 antibody in HepG2 and primary liver cells . HepG2 cells were stained with chimeric 4G6 antibody at the concentration of 16 μg/mL (A) and 8 μg/mL, with Santa Cruz mouse anti-PDC-E2 antibody sc-271534 (C), and chimeric anti-TSHR antibody (12 μg/mL) (D). DAPI was used to stain the nucleus. The primary monkey liver cell slides from Euroimmune Inc., were incubated with 10, 2.5, and 0.625 μg/mL of chimeric 4G6 antibody (E–G).

Article Snippet: The 4G6 antibody demonstrated a cytoplasmic staining pattern identical to that observed with a commercial mouse monoclonal anti-PDC-E2 antibody from Santa Cruz.

Techniques: Immunostaining, Staining, Concentration Assay, Incubation